Coding

Part:BBa_K1808009:Design

Designed by: Remsha Afzal   Group: iGEM15_TrinityCollegeDublin   (2015-09-16)


CYTOCHROME P450 REDUCTASE HIS TAGGED


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 962
    Illegal AgeI site found at 1687
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1834


Design Notes

This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.


Source

Artemisia annua

References